全文获取类型
收费全文 | 1068篇 |
免费 | 31篇 |
国内免费 | 40篇 |
专业分类
林业 | 92篇 |
农学 | 49篇 |
基础科学 | 44篇 |
79篇 | |
综合类 | 425篇 |
农作物 | 21篇 |
水产渔业 | 26篇 |
畜牧兽医 | 303篇 |
园艺 | 88篇 |
植物保护 | 12篇 |
出版年
2024年 | 3篇 |
2023年 | 13篇 |
2022年 | 15篇 |
2021年 | 36篇 |
2020年 | 22篇 |
2019年 | 21篇 |
2018年 | 14篇 |
2017年 | 26篇 |
2016年 | 42篇 |
2015年 | 34篇 |
2014年 | 57篇 |
2013年 | 55篇 |
2012年 | 79篇 |
2011年 | 85篇 |
2010年 | 67篇 |
2009年 | 105篇 |
2008年 | 78篇 |
2007年 | 94篇 |
2006年 | 59篇 |
2005年 | 48篇 |
2004年 | 33篇 |
2003年 | 19篇 |
2002年 | 24篇 |
2001年 | 20篇 |
2000年 | 11篇 |
1999年 | 16篇 |
1998年 | 8篇 |
1997年 | 10篇 |
1996年 | 10篇 |
1995年 | 7篇 |
1994年 | 3篇 |
1993年 | 6篇 |
1992年 | 4篇 |
1991年 | 7篇 |
1990年 | 3篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1955年 | 1篇 |
排序方式: 共有1139条查询结果,搜索用时 15 毫秒
1.
2.
l-proline (Pro) is a precursor of ornithine, which is converted into polyamines via ornithine decarboxylase (ODC). Polyamines plays a key role in the proliferation of intestinal epithelial cells. The study investigated the effect of Pro on polyamine metabolism and cell proliferation on porcine enterocytes in vivo and in vitro. Twenty-four Huanjiang mini-pigs were randomly assigned into 1 of 3 groups and fed a basal diet that contained 0.77% alanine (Ala, iso-nitrogenous control), 1% Pro or 1% Pro + 0.0167% α-difluoromethylornithine (DFMO) from d 15 to 70 of gestation. The fetal body weight and number of fetuses per litter were determined, and the small and large intestines were obtained on d 70 ± 1.78 of gestation. The in vitro study was performed in intestinal porcine epithelial (IPEC-J2) cells cultured in Dulbecco''s modified Eagle medium-high glucose (DMEM-H) containing 0 μmol/L Pro, 400 μmol/L Pro, or 400 μmol/L Pro + 10 mmol/L DFMO for 4 d. The results showed that maternal dietary supplementation with 1% Pro increased fetal weight; the protein and DNA concentrations of the fetal small intestine; and mRNA levels for potassium voltage-gated channel, shaker-related subfamily, member 1 (Kv1.1) in the fetal small and large intestines (P < 0.05). Supplementing Pro to either gilts or IPEC-J2 cells increased ODC protein abundances and polyamine concentrations in the fetal intestines and IPEC-J2 cells (P < 0.05). In comparison with the Pro group, the combined administration of Pro and DFMO reduced the expression of ODC protein and spermine concentration in the fetal intestine, as well as the concentrations of putrescine, spermidine and spermine in IPEC-J2 cells (P < 0.05). Meanwhile, the percentage of cells in the S-phase and the mRNA levels of proto-oncogenes c-fos and c-myc were increased in response to Pro supplementation, whereas depletion of cellular polyamines with DFMO increased tumor protein p53 (p53) mRNA levels (P < 0.05). Taken together, dietary supplementation with Pro improved fetal pig growth and intestinal epithelial cell proliferation via enhancing polyamine synthesis. 相似文献
3.
4.
Based on the perspective of ecological concept,this study first interpreted the background of ecological civilization construction,and combed the relationship between ecological concept and small town planning.Second,it expounded the ecological problems of natural resources,economic development,landscape system,social humanities and other aspects in the construction of contemporary small towns.According to the study,the construction of small towns based on the ecological concept should establish the concept of "ecology first",practice "multiple compliance",coordinate the relationship with other planning,make reasonable planning and design in the aspects of town scale,functional orientation,structural form,road traffic,landscape style and features,humanistic characteristics,and strengthen the implementation,supervision and management of planning.Finally,summary and outlook was made,hoping that the study will be of reference value and guidance to the planning and construction of small towns. 相似文献
5.
6.
7.
益生菌是一类对宿主机体有益、安全无害的活微生物,代替抗生素和疫苗广泛用于畜禽细菌性肠炎的防治。益生菌主要通过与病原菌竞争黏附位点、分泌抑菌物质、加固肠道黏膜屏障、调节肠道免疫应答等抑制病原菌增殖,缓解肠道炎症,防治细菌性肠炎的发生。本文对益生菌的作用机制进行了详细阐述,为益生菌的研究和应用提供参考。 相似文献
8.
阔叶树小径材热压干燥工艺的研究 总被引:2,自引:0,他引:2
通过对荷木和米槠两种华南地区常用阔叶树小径材薄板材热压干燥工艺的研究,探讨热压干燥工艺参数对小径材板材干燥质量(包括密度,厚度压缩率,尺寸稳定性及部分力学性能)的影响,试验结果表明,热压干燥工艺可以大大改善小径材的物理力学性能。 相似文献
9.
AIM: To investigate the expression of Grb2-associated binding protein 2 (Gab2) in human osteosarcoma cells and its relationship with the invasion and metastases of human osteosarcoma cells. METHODS: The technique of small RNA interference was used to transfect human osteosarcoma U2-OS cell lines. Western blotting and RT-PCR were used to detect the protein and mRNA expression of Gab2 in transfected U2-OS cells. After transfection, through chemotaxis and invasion assays in vitro, the cell migration and invasion abilities were detected. RESULTS: After transfection, the expression of Gab2 at mRNA and protein levels in Gab2 siRNA transfected cells (SiGab2/U2-OS) was lower than that in scrambled siRNA transfected cells (Scr/U2-OS) and U2-OS cells. After stimulation with epidermal growth factor (EGF) at concentration of 10 μg/L, the migration SiGab2/U2-OS cells was significantly less than Scr/U2-OS cells and U2-OS cells (P<0.01). The number of invasion cells of SiGab2/U2-OS group was significantly lower than the other 2 control groups (P<0.01). CONCLUSION: Inhibition of Gab2 expression obviously attenuates the migration and invasion abilities of human osteosarcoma U2-OS cell line. 相似文献
10.
Vegetation dynamics in previously burnt shrublands dominated by gorse (Ulex gallii) in north-west Spain were studied during 4 years under different grazing managements with sheep (Gallega breed) and goats
(Cashmere or local breed). Initially (Period 1), treatments consisted of sheep or goat grazing on four plots (two replicates).
After two grazing seasons, the plots were split in two and the treatments were reversed in four of the subplots; sheep were
placed in subplots previously grazed by goats and vice versa (Period 2). The results from Period 1 showed that goats control
the regrowth of gorse (from 19.6 to 14.2% of cover) more effectively than sheep (17.8 to 27.0% cover; P < 0.05), favouring the increase in herbaceous plants (from 21.5 to 42.1% of cover under goat grazing and from 22.1 to 26.6%
under sheep grazing; P < 0.01). In Period 2, after changing treatments, there was a clear effect of the previous management (P < 0.01), conditioning the dynamics of the different plant components. Where the same animal species were maintained for 4 years,
the differences between sheep or goat grazing on gorse and herbaceous plant dynamics increased (24.4% of gorse and 35.2% of
herbaceous plant cover under goat grazing vs 43.8% of gorse cover and 19.1% of herbaceous plant cover under sheep grazing).
Animal species change buffered the differences between sheep and goats during the second Period. 相似文献